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JoVE Journal Neuroscience
Pålidelig Identifikation af Living dopaminerge neuroner i midthjernen Cultures Brug RNA Sequencing og TH-promotor-drevet eGFP Expression
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Pålidelig Identifikation af Living dopaminerge neuroner i midthjernen Cultures Brug RNA Sequencing og TH-promotor-drevet eGFP Expression

Article DOI: 10.3791/54981-v • 10:54 min • February 10th, 2017
February 10th, 2017 •
1, 1, 1, 1, 1, 1, 1, 1
1Division of Biology and Biological Engineering, California Institute of Technology (Caltech)

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Ved Parkinsons sygdom (PD), sorte substans (SNC) dopaminerge neuroner degenerere, hvilket fører til motorisk dysfunktion. Her rapporterer vi en protokol til dyrkning ventrale midthjernen neuroner fra en mus, der udtrykker eGFP drevet af en tyrosinhydroxylase (TH) promotorsekvens, høst individuelle fluorescerende neuroner fra kulturerne, og måling af deres transkriptom anvendelse af RNA-seq.

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Neuroscience encellede RNA-sekventering cellehøst midthjernen kulturer dopaminerge neuroner Parkinsons sygdom
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