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Vurdere Neurodegenerative fænotyper i<em> Drosophila</em> Dopaminerge neuroner ved at klatre assays og Whole Brain-immunfarvning
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining
DOI:

09:17 min

April 24, 2013

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Chapters

  • 00:05Title
  • 01:10Startle-induced Negative Geotaxis Assay
  • 03:14Negative Geotaxis Assay Data Analysis
  • 04:07Dissecting Adult Drosophila Brains for Whole Mounts
  • 05:14Tyrosine Hydroxylase Immunofluorescence of Whole Mount Brains
  • 08:10Investigating the Stress-protective Nrf2 Pathway in a Drosophila Model of PD
  • 08:56Conclusion

Summary

Automatic Translation

Her beskriver vi to analyser, som er blevet oprettet for at studere aldersafhængig neurodegeneration af dopaminerge (DA) neuroner i<em> Drosophila</em>: Klatring / startle-induceret negativ geotaxis assay, der gør det muligt at studere de funktionelle virkninger af DA neuroner degeneration og tyrosinhydroxylase immunfarvning som bruges til at identificere og tælle DA neuroner i hele hjernen mounts.

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