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JoVE Journal Neuroscience
I Utero Elektroporation Approaches to Undersøgelse ophidselse af neuronale subpopulationer og Single-celle Connectivity
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I Utero Elektroporation Approaches to Undersøgelse ophidselse af neuronale subpopulationer og Single-celle Connectivity

Article DOI: 10.3791/55139-v • 10:49 min • February 15th, 2017
February 15th, 2017 •
1, 2, 2, 1
1Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM)

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Dette håndskrift indeholder protokoller, der bruger i livmoderen elektroporation (IUE) til at beskrive den strukturelle konnektivitet af neuroner på encellede niveau og ophidselse af fluorescens-mærkede neuroner. Histologi anvendes til at karakterisere dendritiske og axonale projektioner. Helcelle-optagelse i akutte skiver anvendes til at undersøge ophidselse.

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Neuroscience hjerne udvikling cortex elektrofysiologi corpus callosum elektroporering
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