Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs
 

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Article DOI: 10.3791/55239-v 11:24 min June 12th, 2017
June 12th, 2017

Chapters

Summary

Gene deletion mutants generated through homologous recombination are the gold standard for gene function studies. The OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) method for rapid generation of deletion constructs is described. Agrobacterium mediated fungal transformation follows. Finally, a PCR based confirmation method of gene deletions in fungal transformants is presented.

Tags

Gene Deletion Mutants Fungi Functional Genetics OSCAR Deletion Constructs Plasmids Homologous Recombination System Protist Vermilion Cells DelsGate System Protoplast Transformation Agrobacterium Primer Design Tool
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter