Un Tailored HPLC purificazione protocollo che i rendimenti di elevata purezza beta-amiloide 42 e beta amiloide 40 peptidi, in grado di Oligomer Formazione


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Warner, C. J., Dutta, S., Foley, A. R., Raskatov, J. A. A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation. J. Vis. Exp. (121), e55482, doi:10.3791/55482 (2017).

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Name Company Catalog Number Comments
Agilent 1260 Infinity II quarternary pump Agilent G7111B http://www.agilent.com/en-us/products/liquid-chromatography/lc-pumps-vacuum-degassers/1260-infinity-ii-quaternary-pump
Agilent 1260 Infinity II Dual variable wavelength detector Agilent G7114A http://www.agilent.com/en-us/products/liquid-chromatography/lc-detectors/1260-infinity-ii-variable-wavelength-detector
Agilent 1260 Infinity II Manual Injector fitted with 10 mL stainless steel sample loop Agilent 0101-1232 http://www.agilent.com/en-us/products/liquid-chromatography/lc-injection-systems/1260-infinity-ii-manual-injector
Agilent 1260 Infinity II Manual Injector fitted with 20 µL stainless steel sample loop Agilent G1328C http://www.agilent.com/en-us/products/liquid-chromatography/lc-injection-systems/1260-infinity-ii-manual-injector
Ring Stand Mounting Bracket Agilent 1400-3166
Agilent PLRP-S 300 Å 5 µm 4.6 x 250 mm (Analytical) Agilent PL1512-5501 http://www.agilent.com/en-us/products/liquid-chromatography/lc-columns/biomolecule-separations/plrp-s-for-biomolecules#features
Aβ42 or Aβ40 peptide Synthesized in-house using a CEM liberty automated peptide synthesizer.
Ammonium Hydroxide (NH4OH, 28% solution) Fisher Scientific A669-500
Acetonitrile Fisher Scientific A998-4
HPLC grade water Fisher Scientific W5-4
Falcon 50 mL conical centrifuge tube Fisher Scientific 14-954-49A
Supelco PEEK Fitting One-piece fingertight, pkg of 5 ea Sigma-Aldrich Z227250
Normject 5 cc sterile syringe Fisher Scientific 1481729
16 Gauge SS Needle Rheodyne 3725-086



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