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Journal / Neuroscience / 测定在运动神经元样NSC-34细胞中实时测量核细胞质运输…
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JoVE Journal Neuroscience
测定在运动神经元样NSC-34细胞中实时测量核细胞质运输
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测定在运动神经元样NSC-34细胞中实时测量核细胞质运输

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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该方案描述了一种通过量化NLS-NES-GFP蛋白的核进口的实时变化来敏感地测量运动神经元样NSC-34细胞内的核胞质转运速率的方法。

Tags

神经科学，第123期，核细胞质转运，GFP，NSC-34细胞，C9orf72，ALS
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