JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
电机神经切断和时间推移成像胶质细胞行为在现场斑马鱼
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
电机神经切断和时间推移成像胶质细胞行为在现场斑马鱼
DOI:
10.3791/50621-v
•
09:05 min
•
June 20, 2013
•
Gwendolyn M. Lewis
,
Sarah Kucenas
1
Department of Biology
,
University of Virginia
Chapters
00:05
Title
01:18
Preparation and Mounting of Zebrafish Embryos
03:10
Laser Calibration and Testing
04:35
Nerve Transection Using Laser Ablation and Time-lapse Confocal Imaging of Glial Cell Behaviors
06:39
Representative Laser Ablation Results
08:39
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
虽然周围神经系统(PNS)能显着损伤后的修复,治理这种现象的细胞和分子机制知之甚少。使用现场,转基因斑马鱼和一个可重复的神经切断术实验,我们可以研究动态神经胶质细胞在神经变性和再生行为。
Tags
Motor Nerve Transection
Time-lapse Imaging
Glial Cell Behaviors
Zebrafish
Peripheral Nervous System
Regeneration
Axon
Schwann Cells
Perineurium
Axotomy
In Vivo Imaging
Genetic Manipulation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在哺乳动物的中枢神经系统视神经横断后的实验操作方法
在发展中国家斑马鱼的前脑的神经祖细胞克隆相关的定时实时成像
实时成像背根轴突后根切断术
急性片的成年小鼠前脑的神经细胞迁移的延时成像
成神经细胞迁移的小鼠急性脑片
在体内
电穿孔产后和时间推移成像
诱导星形胶质细胞受体的可塑性由神经元的放电频率操作
在斑马鱼幼虫脊髓横断
老鼠耳蜗外植体的长期时间推移成像
来自胚胎GFP-表达小鼠的Ex Vivo Oculo运动切片培养,用于对Oculo运动神经外生长进行时移成像
坐骨神经粉碎损伤大鼠模型中功能评估的3D运动学分析
Read Article