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Journal / Cancer Research / Ex Vivo Imagem de linfócitos de T CD8 residente…
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Cancer Research

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JoVE Journal Cancer Research
Ex Vivo Imagem de linfócitos de T CD8 residente no Tumor de pulmão humano fatias utilizando microscopia Confocal
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Ex Vivo Imagem de linfócitos de T CD8 residente no Tumor de pulmão humano fatias utilizando microscopia Confocal

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Este protocolo descreve um método de imagem residente tumor infiltrando T CD8 células rotulado com anticorpos fluorescente acoplados dentro de fatias de tumor de pulmão humano. Esta técnica permite análises em tempo real de migração de células T CD8 usando microscopia confocal.

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Cancer Research edição 130 imunologia de Tumor humanos tumor de pulmão fatias de organotypic células T migração fluorescência microscopia confocal,
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