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Induction of Hypoxia in Living Frog and Zebrafish Embryos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Induction of Hypoxia in Living Frog and Zebrafish Embryos
DOI:

08:01 min

June 26, 2017

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Chapters

  • 00:05Title
  • 00:46Induction of Hypoxia In Vivo
  • 02:40Induction of Hypoxia in Embryos and Collection of Tissue to Monitor HIF-1α Levels
  • 04:19Incubation of Embryos in Hypoxia for EdU Incorporation
  • 05:13Results: The Effects of Hypoxia on HIF-1α and on Cell Proliferation
  • 06:42Conclusion

Summary

Automatic Translation

We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.

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