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JoVE Journal Cancer Research
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
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Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

Article DOI: 10.3791/55890-v • 09:03 min • October 16th, 2017
October 16th, 2017 •
1, 1, 1
1Department of Pathology and Laboratory Medicine, Weill Cornell Medicine

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Summary
Automatic Translation

We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.

Tags

Identification Characterization Metastatic Factors Gene Transfer RIP-Tag RIP-tva Mouse Model Cancer Metastasis Therapeutic Targets Somatic Gene Transfer Pancreatic Beta Cell Precursor Lesions Virus Producing DF1 Cells Viral Supernatant Low-speed Centrifugation Ultracentrifuge Tubes Viral Suspension Laboratory Film
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