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JoVE Journal Neuroscience
Quadruple Immunostaining av Olfactory Bulb för Visualisering av Olfactory Sensory Axon Molecular Identity Codes
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Quadruple Immunostaining av Olfactory Bulb för Visualisering av Olfactory Sensory Axon Molecular Identity Codes

Article DOI: 10.3791/55893-v • 06:32 min • June 5th, 2017
June 5th, 2017 •
1, 1,2, 1,3
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST)

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Olfaktoriska sensoriska neuroner uttrycker ett brett utbud av axon-sorteringsmolekyler för att upprätta en lämplig neuralkrets. Detta protokoll beskriver en immunohistokemisk färgmetod för att visualisera kombinatoriska uttryck av axonsorteringsmolekyler vid axonterminalerna av olfaktoriska sensoriska neuroner.

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Neurovetenskap Utgåva 124 olfaktoriskt system immunhistokemi genuttryck axonvägledning neural kretsbildning olfaktoriska sensoriska neuroner
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