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JoVE Journal Cancer Research
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
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Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

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Summary
Automatic Translation

Targeted gene editing using CRISPR/Cas9 has greatly facilitated the understanding of the biological functions of genes. Here, we utilize the CRISPR/Cas9 methodology to model calreticulin mutations in cytokine-dependent hematopoietic cells in order to study their oncogenic activity.

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CRISPR/Cas9 Gene Editing Mutant Calreticulin Oncogenic Activity Cytokine Dependent Hematopoietic Cells Hematological Malignancies Driver Mutations Biological Function CRISPR/Cas9 Gene Editing System LentiGuide-Puro Vector Digestor Reaction BsmB1 Restriction Enzyme Dephosphorylate Phosphatase Reaction Buffer Phosphatase Enzyme Ligation Mixture
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