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Journal / Biochemistry / Kombinere røntgenkrystallografi med lille vinkel…
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Biochemistry

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JoVE Journal Biochemistry
Kombinere røntgenkrystallografi med lille vinkel X-Ray spredning til Model ustruktureret regioner af Nsa1 fra S. Cerevisiae
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Kombinere røntgenkrystallografi med lille vinkel X-Ray spredning til Model ustruktureret regioner af Nsa1 fra S. Cerevisiae

Article DOI: 10.3791/56953-v • 09:15 min • January 10th, 2018
January 10th, 2018 •
1, 1, 1
1Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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Denne metode beskriver kloning, udtryk og oprensning af rekombinante Nsa1 for Strukturbestemmelse af røntgenkrystallografi og små-vinkel X-ray spredning (SAXSA), og er gældende for hybrid strukturel analyse af andre proteiner der indeholder begge bestilt og uordnede domæner.

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Biokemi sag 131 røntgenkrystallografi SAXSA Hybrid metoder Protein oprensning WD40 domæne begrænset proteolyse ribosomet forsamling
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