JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从
s 酿酒酵母
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from
S. Cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
结合 x 射线晶体学与小角度 x 射线散射到模型的非结构化区域的 Nsa1 从
s 酿酒酵母
DOI:
10.3791/56953-v
•
09:15 min
•
January 10, 2018
•
Yu-Hua Lo
,
Monica C. Pillon
,
Robin E. Stanley
1
Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services
,
National Institutes of Health
Chapters
00:05
Title
00:47
Nsa1 (Nop7 Associated 1) Protein Purification
03:43
Proteolytic Screening
05:14
Small-Angle X-Ray Scattering (SAXS) Data Processing, Comparison of Concentration Series, and Ensemble Modeling
07:31
Results: Modeling the Disordered C-Terminus Region of Nsa1
08:37
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该方法描述了用 x 射线晶体学和小角 x 射线散射 (SAXS) 进行结构测定的重组 Nsa1 的克隆、表达和纯化, 适用于其他蛋白质的混合结构分析包含有序和无序的域。
Tags
X-ray Crystallography
Small Angle X-ray Scattering (SAXS)
Structural Analysis
Protein Structure
Recombinant Protein
Unstructured Regions
Nsa1
S. Cerevisiae
Hybrid Structural Analysis
Protein Function
Protein Dynamics
Protein Aggregation
Purification
Affinity Chromatography
Gel Filtration
SDS-PAGE
TEV Protease
Centrifugal Filtration
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
用 X 射线晶体学和生物物理技术表征免疫球蛋白折叠的糖蛋白
用于膜蛋白结构分析和
从头
建模的小角中子散射实验中的对比匹配洗涤剂
小角度 x 射线散射溶液中大分子的结构研究
离子交换色谱 (IEX) 与多角度光散射 (ALS) 相结合, 用于蛋白质分离和表征
蛋白质的表征(按尺寸-排除色谱与多角度光散射相结合(SEC-MALS)
用于大分子 X 射线晶体学的一体式样品支架,具有最小的背景散射
硅氮化物膜的细胞培养和同步加速器X射线荧光纳米分析的冷冻制备
X射线晶体学研究热木体马里蒂玛M42氨基肽
TmPep1050的寡聚态过渡
金刚石光源的固定目标串行数据收集
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from
S. cerevisiae
Cells
Read Article