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Immunology and Infection

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JoVE Journal Immunology and Infection
Winning van Hemocytes van Drosophila melanogaster larven voor microbiële infectie en analyse
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Winning van Hemocytes van Drosophila melanogaster larven voor microbiële infectie en analyse

Article DOI: 10.3791/57077-v • 08:54 min • May 24th, 2018
May 24th, 2018 •
1, 2, 1
1School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine, Washington State University

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Deze methode laat zien hoe om te visualiseren pathogen invasie in insect cellen met driedimensionale (3D) modellen. Hemocytes van Drosophila larven waren besmet met virale of bacteriële pathogenen, ex vivo of in vivo. Besmette hemocytes werden vervolgens vast en gekleurd voor imaging met een confocal microscoop en de daaropvolgende 3D cellulaire wederopbouw.

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Immunologie en infecties probleem 135 Pathogen invasie hemocyte extractie confocal microscopie Coxiella burnetii Listeria monocytogenes 6 IIV6-ongewervelde iriserende virus,
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