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Immunology and Infection

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JoVE Journal Immunology and Infection
Utvinning av Hemocytes fra Drosophila melanogaster larver for mikrobielle infeksjoner og analyse
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Utvinning av Hemocytes fra Drosophila melanogaster larver for mikrobielle infeksjoner og analyse

Article DOI: 10.3791/57077-v • 08:54 min • May 24th, 2018
May 24th, 2018 •
1, 2, 1
1School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine, Washington State University

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Denne metoden viser hvordan du visualisere patogen invasjonen i insekt celler med tredimensjonale (3D) modeller. Hemocytes fra Drosophila Larvene var infisert med virus eller bakterielle patogener, ex vivo eller i vivo. Infiserte hemocytes ble deretter fast og farget for bildebehandling med AC confocal mikroskop og påfølgende 3D cellular rekonstruksjon.

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Immunologi og infeksjon problemet 135 patogen invasjon hemocyte utvinning AC confocal mikroskopi Coxiella burnetii Listeria monocytogenes virvelløse iriserende virus-6 IIV6
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