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Ekstraktion af Hemocytes fra Drosophila melanogaster larver for mikrobiel infektion og analyse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

Ekstraktion af Hemocytes fra Drosophila melanogaster larver for mikrobiel infektion og analyse

DOI:
10.3791/57077-v

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08:54 min

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May 24, 2018

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, ,
1School of Molecular Biosciences, College of Veterinary Medicine,Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine,Washington State University

Chapters

  • 00:05Title
  • 00:45Ex Vivo Infection
  • 01:48Hemolymph Extraction
  • 03:11In Vivo Infection
  • 04:08Hemolymph Extraction and Hemocyte Plating
  • 04:35Confocal Imaging
  • 05:443D Model Reconstruction
  • 06:58Results: Dynamic Visualization of Drosophila Hemocytes
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

Denne metode viser, hvordan man visualisere patogen invasion i insekt celler med tre-dimensionelle (3D) modeller. Hemocytes fra Drosophila larver var smittet med virale eller bakterielle patogener, enten ex vivo eller i vivo. Inficerede hemocytes blev derefter fast og farvet for billeddannelse med en Konfokal mikroskop og efterfølgende 3D cellulære genopbygning.

Tags

HemocytesDrosophila MelanogasterMicrobial InfectionImmune ResponseEx Vivo InfectionConfocal Microscopy3D Model ReconstructionCapillary TubeNanoinjectorHemolymphHemocyte IsolationDrosophila Hemocyte Isolating Medium (DHIM)AnesthesiaGrape Juice Agar PlateYeast Paste

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