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Journal / Biology / Visualisering af DNA jordpakning i cyanobakterier …
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Biology

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Visualisering af DNA jordpakning i cyanobakterier af høj spænding Cryo-elektron tomografi
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Visualisering af DNA jordpakning i cyanobakterier af høj spænding Cryo-elektron tomografi

Article DOI: 10.3791/57197-v • 09:47 min • July 17th, 2018
July 17th, 2018 •
1, 2
1National Institute for Physiological Sciences, 2Graduate School of Science and Engineering, Saitama University

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Denne protokol beskriver hvordan man visualisere den forbigående DNA komprimering i cyanobakterier. Synkron dyrkning, overvågning af Fluorescens mikroskopi, Hurtig nedfrysning og høj spænding cryo-elektron tomografi bruges. En protokol for disse metoder præsenteres, og fremtidige programmer og udviklingen diskuteres.

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Biologi sag 137 synkron kultur Fluorescens mikroskopi Hurtig nedfrysning høj spænding cryo-elektron tomografi segmentering cyanobakterier DNA jordpakning celledeling tyk model
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