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Chapters
- 00:04Title
- 01:00Tissue Sectioning
- 02:55Microdissection
- 06:20Extraction and LC/MS Analysis
- 08:14Results: Spatially-resolved Quantification of Ethambutol in Pulmonary Lesions by LCM-LC/MS
- 09:19Conclusion
Here, we describe a protocol using laser capture microdissection coupled with LC/MS analysis to spatially-quantify drug distributions within pulmonary tuberculosis granulomas. The approach has broad applicability to quantifying drug concentrations within tissues at high spatial detail.
