Journal
/
Biology
/
An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

DOI:
10.3791/57604-v

•

05:12 min

•

April 16, 2018

•

, , , , , , ,
1Department of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, Shanghai Key Clinical Disciplines of otorhinolaryngology,Fudan University, 2Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Department of Medical Microbiology and Parasitology, School of Basic Medical Science,Shanghai Medical College of Fudan University, 3Department of Dermatology, Huashan Hospital,Fudan University

Chapters

  • 00:04Title
  • 00:40Biofilm Formation and Light Irradiation
  • 02:17Photodynamic Therapy Analysis
  • 04:00Results: Representative Biofilm Viability Analyses
  • 04:33Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes a protocol to study the antimicrobial effect of 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) on a Staphylococcus aureus biofilm. This protocol can be used to develop an in vitro model to study the treatment of bacterial biofilms with PDT in the future.

Tags

5-aminolevulinic AcidPhotodynamic TherapyStaphylococcus AureusBiofilmIn Vitro ModelAnti-microbial EffectBacterial InfectionCell CultureLight-based TreatmentViable Bacteria Count

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

Christopher Hughes: An in vitro model for the Study of Angiogenesis (Interview)

Computer-Generated Animal Model Stimuli

An Alternant Method to the Traditional NASA Hindlimb Unloading Model in Mice

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research

Read Article