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The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster
JoVE Journal
Genetics
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JoVE Journal Genetics
The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster

The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster

DOI:
10.3791/57788-v

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07:21 min

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June 08, 2018

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, , ,
1Department of Biological Sciences,University of Alabama

Chapters

  • 00:04Title
  • 00:37Fly Collection and Maintenance
  • 03:27Exercise Protocol
  • 05:29Results: Exercise and Diet Effects on Protein-levels and Locomotion
  • 06:51Conclusion

Summary

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The TreadWheel uses rotational motion to gently induce exercise in adult Drosophila melanogaster by exploiting flies' innate, negative geotaxis. It allows for analysis of the interactions between exercise and factors, such as genotype, sex, and diet, and their effect on physiological and molecular assays to assess metabolic health.

Tags

Drosophila MelanogasterTreadWheelInterval TrainingExerciseMetabolic ResearchFirst Instar LarvaeApple Juice Agar PlatesYeast PasteLaying ChambersExperimental ConditionsGenotypesPupaeEclosion

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