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Biochemistry

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JoVE Journal Biochemistry
Efficiënte reiniging en LC-MS/MS-based Assay ontwikkeling voor tien-elf translocatie-2 5-Methylcytosine Dioxygenase
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Efficiënte reiniging en LC-MS/MS-based Assay ontwikkeling voor tien-elf translocatie-2 5-Methylcytosine Dioxygenase

Article DOI: 10.3791/57798-v • 10:34 min • October 15th, 2018
October 15th, 2018 •
*1, *1, *1, 1, 1
1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City
* These authors contributed equally

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Hier presenteren we een protocol voor een efficiënte Macrostap zuivering van de actieve niet-gelabelde mens tien-elf translocatie-2 (TET2) 5-methylcytosine-dioxygenase met behulp van ionenwisselingschromatografie- en haar kwantitatieve analyse met behulp van een vloeibare chromatografie-tandem-massa spectrometrie (LC-MS/MS)-gebaseerde aanpak.

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Biochemie kwestie 140 tien-elf translocatie Demethylase leukemie Dioxygenase LC-MS/MS epigenetica transcriptieregulatie
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