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Journal / Immunology and Infection / Långsiktiga In Vivo -spårning av inflammatoriska…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Långsiktiga In Vivo -spårning av inflammatoriska celler dynamiken inom Drosophila puppor
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Långsiktiga In Vivo -spårning av inflammatoriska celler dynamiken inom Drosophila puppor

Article DOI: 10.3791/57871-v • 09:26 min • June 14th, 2018
June 14th, 2018 •
1,2, 1, 3, 1,4
1School of Biochemistry, Biomedical Sciences, University of Bristol, 2School of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, 3MRC Centre for Inflammation Research, University of Edinburgh, Queens Medical Research Institute, 4School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences, University of Bristol

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Här presenterar vi ett protokoll för live-imaging såret reparation och den associera inflammatoriska reaktionen på plats och tid högupplösta i vivo. Denna metod använder Drosophila Pupp utvecklingsstadiet att möjliggöra långsiktiga imaging och spårning av viss cellpopulationer över tid och är kompatibel med effektiv RNAi-medierad genen inaktivering.

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Immunologi och infektion fråga 136 sår läkning inflammation medfödd immunitet Drosophila melanogaster Drosophila puppa hemocyte cellmigration live-imaging laser ablation konfokalmikroskopi photoconversion
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