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Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
DOI:

14:26 min

April 06, 2019

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Chapters

  • 00:00Title
  • 01:41Protocol
  • 09:25Results
  • 13:16Conclusion

Summary

Automatic Translation

This method of trypanosome separation from blood depends on their surface charge being less negative than mammalian blood cells. Infected blood is placed and treated on an anion-exchanger column. This method, the most fitting diagnostic for African trypanosomiasis, provides purified parasites for immunological, biological, biochemical, pharmaceutical and molecular biology investigations.

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