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Chapters
- 00:00Title
- 02:17Procedure 1: Insertion of the Line for Intraintestinal Administration
- 03:57Procedure 2: Inferior Vena Cava Catheterization
- 06:44Procedure 3: Portal Vein Catheterization
- 10:46Procedure 4: Portal Vein Blood Sampling
- 11:22Procedure 5: Inferior Vena Cava Blood Sampling
- 11:51Procedure 6: Administration of Permeability Marker
- 12:59Procedure 7: Calculation of Hepatic Clearance
- 13:20Results
- 14:16Conclusion
The access of nutrients, microbiota metabolites and medicines to the circulation is controlled by the gut-blood barrier (GBB). We describe a direct method for measuring the GBB permeability in vivo, which, in contrast to commonly used indirect methods, is virtually not affected by liver and kidney functions.
