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Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
JoVE Journal
Genetics
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JoVE Journal Genetics
Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
DOI:

08:01 min

November 20, 2018

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Chapters

  • 00:04Title
  • 01:02Southern Blotting Screening
  • 04:40Results: Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells
  • 06:07Conclusion

Summary

Automatic Translation

Here, we present a detailed protocol for identifying homologous recombination events that occurred in mouse embryonic stem cells using Southern blotting and/or PCR. This method is exemplified by the generation of nonmuscle myosin II genetic replacement mouse models using traditional embryonic stem cell-based homologous recombination-mediated targeting technology.

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