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Journal / Genetics / Identificatie van homologe recombinatie…
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Genetics

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JoVE Journal Genetics
Identificatie van homologe recombinatie gebeurtenissen in muis embryonale stamcellen met behulp van Southern Blotting en de Kettingreactie van de Polymerase
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Identificatie van homologe recombinatie gebeurtenissen in muis embryonale stamcellen met behulp van Southern Blotting en de Kettingreactie van de Polymerase

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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Hier presenteren we een gedetailleerd protocol voor het identificeren van homologe recombinatie gebeurtenissen die hebben plaatsgevonden in muis embryonale stamcellen met behulp van Southern blotting en/of PCR. Deze methode wordt geïllustreerd door de generatie van nonmuscle myosin II genetische vervanging Muismodellen met behulp van traditionele embryonale stamcel gebaseerde homologe recombinatie-gemedieerde targeting technologie.

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Genetica kwestie 141 homologe recombinatie gene richt Southern blotting PCR muis embryonale stamcellen sonde primer genetische vervanging knock-out/knock-in Myh9 gen genomic DNA
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