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JoVE Journal Cancer Research
Utredning av genetiska beroenden med CRISPR-Cas9-baserade konkurrens analyser
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Utredning av genetiska beroenden med CRISPR-Cas9-baserade konkurrens analyser

Article DOI: 10.3791/58710-v • 11:05 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1, 2, 3, 1
1Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute, 2Blood Research Institute, Blood Center of Wisconsin, 3Department of Bioengineering, University of California, San Diego

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Detta manuskript beskriver en klustrad regelbundet mellanliggande kort Palindromic upprepas (CRISPR) CRISPR-Cas9-baserade metod för enkel och snabb undersökning av flera kandidatgener i akut myeloisk leukemi (AML) cellproliferation i roll parallellt. Denna teknik är skalbar och kan appliceras i andra cancer cellinjer samt.

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Cancerforskning fråga 143 AML CRISPR-Cas9 hög genomströmning assay konkurrenskraftig tillväxt assay DOT1L leukemi cancer
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