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Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
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JoVE Journal Biology
Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

DOI:
10.3791/58832-v

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09:45 min

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January 20, 2019

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,
1Department of Basic Sciences,Loma Linda University

Chapters

  • 00:04Title
  • 00:48Dissection and Isolation of Cerebral Artery
  • 02:34Preparation of Endothelial Tube and Superfusion
  • 05:44Simultaneous Measurement of [Ca2+]i and Vm
  • 08:36Results: Simultaneous [Ca2+]i and Vm Recordings
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

Demonstrated here are protocols for (1) freshly isolating intact cerebral endothelial "tubes" and (2) simultaneous measurements of endothelial calcium and membrane potential during endothelium-derived hyperpolarization. Further, these methods allow for pharmacological tuning of endothelial cell calcium and electrical signaling as individual or interactive experimental variables.

Tags

Intracellular CalciumMembrane PotentialCerebral EndotheliumCerebral Vascular PhysiologyCerebral Blood Flow RegulationAgingChronic DiseasesMouse Brain IsolationCerebral Artery IsolationEnzymatic DigestionSimultaneous MeasurementPhysiological Conditions

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