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Flerfarvet Flow flowcytometri-baserede kvantificering af mitokondrier og lysosomer i T celler
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Flerfarvet Flow flowcytometri-baserede kvantificering af mitokondrier og lysosomer i T celler

Article DOI: 10.3791/58844-v • 06:22 min • January 9th, 2019
January 9th, 2019 •
1, 1, 1, 1
1Institute of Microbiology and Immunology, National Yang-Ming University

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Denne artikel illustrerer en kraftfuld metode til at anslå mitokondrier eller lysosomer i levende celler. Kombinationen af lysosomet eller mitokondrier specifikke farvestoffer med fluorescently konjugerede antistoffer mod celleoverflademarkører giver mulighed for kvantificering af disse organeller i blandet cellepopulationer, som primær celler høstet fra vævsprøver, ved hjælp af flerfarvet flowcytometri.

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Immunologi og infektion spørgsmålet 143 Flow flowcytometri Multifarve T celle lysosomet mitokondrier organelle-specifikke farvestoffer
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