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Flerfarvet Flow flowcytometri-baserede kvantificering af mitokondrier og lysosomer i T celler
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Immunology and Infection
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JoVE Journal Immunology and Infection
Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Flerfarvet Flow flowcytometri-baserede kvantificering af mitokondrier og lysosomer i T celler

DOI:
10.3791/58844-v

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06:22 min

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January 09, 2019

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, , ,
1Institute of Microbiology and Immunology,National Yang-Ming University

Chapters

  • 00:04Title
  • 00:46Mitochondria and Lysosome Labeling
  • 02:27Flow Cytometric Analysis
  • 04:31Results: Representative Mitochondria and Lysosome Detection
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

Denne artikel illustrerer en kraftfuld metode til at anslå mitokondrier eller lysosomer i levende celler. Kombinationen af lysosomet eller mitokondrier specifikke farvestoffer med fluorescently konjugerede antistoffer mod celleoverflademarkører giver mulighed for kvantificering af disse organeller i blandet cellepopulationer, som primær celler høstet fra vævsprøver, ved hjælp af flerfarvet flowcytometri.

Tags

Flow CytometryMitochondriaLysosomesT CellsOrganelle QuantificationImmuno-metabolismCell LabelingCell StainingFluorescence-activated Cell Sorting (FACS)

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