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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
DOI:

10:57 min

June 06, 2019

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Chapters

  • 00:04Title
  • 00:47Coating Glass Coverslips
  • 02:22Dissociation of Hippocampal and Cortical Tissue
  • 07:13Cell Sorting and Culturing of Purified GABAergic or Glutamatergic Neurons
  • 09:19Results: Cell Culture of Purified Glutamatergic or GABAergic Neurons
  • 10:26Conclusion

Summary

Automatic Translation

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.

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