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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

DOI:
10.3791/58974-v

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10:57 min

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June 06, 2019

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, , , ,
1Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

Chapters

  • 00:04Title
  • 00:47Coating Glass Coverslips
  • 02:22Dissociation of Hippocampal and Cortical Tissue
  • 07:13Cell Sorting and Culturing of Purified GABAergic or Glutamatergic Neurons
  • 09:19Results: Cell Culture of Purified Glutamatergic or GABAergic Neurons
  • 10:26Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.

Tags

Primary Cell CultureGABAergic NeuronsGlutamatergic NeuronsFluorescence-activated Cell SortingNeuronal PhysiologySynapsesNeuronal NetworksPLL CoatingCell Culture BufferBSAPapainFilter Sterilization

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