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Journal / Genetics / 原发性人类 CD4+ T 淋巴细胞低线粒体 DNA 污染的 ATAC-seq…
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Genetics

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JoVE Journal Genetics
原发性人类 CD4+ T 淋巴细胞低线粒体 DNA 污染的 ATAC-seq 检测
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原发性人类 CD4+ T 淋巴细胞低线粒体 DNA 污染的 ATAC-seq 检测

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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在这里, 我们提出了一个协议, 以执行检测转座受接触染色质测序 (ATAC-seq) 激活 CD4 + 人类淋巴细胞。该协议已被修改, 以尽量减少污染线粒体 DNA 读取从50% 到3% 通过引入一个新的裂解缓冲液。

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遗传学 第145期 Epigenomics Ata-seq Tn5 CD4 + 淋巴细胞 细胞核 线粒体 DNA 污染
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