04:19Plating Organoids and Co-culturing with Macrophages
05:27Immunofluorescence Staining of Organoids
07:08Results: Irradiated Epithelial Mammary Organoids were Cultured on Low-Adhesion Plates, Stained for Various Markers, and Co-Cultured with Immune Cells
Organoids developed from mouse mammary glands were irradiated and characterized to assess epithelial traits and interactions with immune cells. Irradiated organoids can be used to better evaluate cell-cell interactions that may lead to tumor cell recruitment in irradiated normal tissue.