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JoVE Journal Neuroscience
Konvertering av Human indusert pluripotent Stem Cells (iPSCs) i funksjonell spinal og skallen motor neurons bruke PiggyBac vektorer
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Konvertering av Human indusert pluripotent Stem Cells (iPSCs) i funksjonell spinal og skallen motor neurons bruke PiggyBac vektorer

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Denne protokollen gir rask og effektiv konvertering av indusert Pluripotent stamceller i motoriske neurons med en spinal eller skallen identitet, ved svangerskap utenfor livmoren av transkripsjon faktorer fra induserbart piggyBac vektorer.

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Nevrovitenskap indusert Pluripotent stamceller motoriske neurons piggyBac differensiering transkripsjon faktorer Phox2a Isl1 Ngn2 Lhx3
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