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利用 PiggyBac 载体将人诱导的多能干细胞 (Ipsc) 转化为功能性脊柱和颅内运动神经元
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Neuroscience
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JoVE Journal Neuroscience
Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
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利用 PiggyBac 载体将人诱导的多能干细胞 (Ipsc) 转化为功能性脊柱和颅内运动神经元

DOI:
10.3791/59321-v

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07:33 min

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May 01, 2019

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, , , , , , ,
1Department of Biology and Biotechnology Charles Darwin,Sapienza University of Rome, Italy, 2Center for Life Nano Science,Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology,The Rockefeller University, USA, 4Department of Physiology and Pharmacology,Sapienza University of Rome, Italy

Chapters

  • 00:04Title
  • 00:54Ngn2, Isl1 and Lhx3 (NIL) and Ngn2, Isl1 and Phox2a (NIP) Induced Pluripotent Stem Cell (iPSC) Line Generation
  • 02:19Antibiotic Selection
  • 03:18Motor Neuron Differentiation
  • 05:12Results: Representative Human iPSC Motor Neuron (MN) Differentiation Analysis
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

该协议允许诱导多能干细胞快速有效地转换为具有脊柱或颅内身份的运动神经元, 通过从诱导的 piggyBac 载体的转录因子的异位表达。

Tags

Human Induced Pluripotent Stem CellsIPSCsSpinal Motor NeuronsCranial Motor NeuronsPiggyBac VectorsMotor Neuron DiseasesAmyotrophic Lateral SclerosisCell DifferentiationTransfectionBlasticidin SelectionDoxycycline InductionRT-PCR

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