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An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
DOI:

09:48 min

September 12, 2019

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Chapters

  • 00:04Title
  • 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
  • 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
  • 03:27Surgical Field Preparation
  • 04:17Laparotomy and Uterine Horn Identification
  • 05:31Myometrial VX2 Cell Inoculation
  • 06:21Surgical Incision Closure and Post-Operative Care
  • 08:03Results: VX2 Tumor Establishment and Characterization
  • 09:09Conclusion

Summary

Automatic Translation

This protocol presents a standardized method to grow VX2 cells in culture and to create an orthotopic VX2 model of endometrial cancer with retroperitoneal lymph node metastases in rabbits. Orthotopic endometrial cancer models are important for the pre-clinical study of novel imaging modalities for the diagnosis of lymph node metastases.

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