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An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
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1Institute of Medical Science,University of Toronto, 2Department of Obstetrics and Gynecology,University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute,University Health Network, 4Department of Pathology, Princess Margaret Cancer Center,University Health Network, 5Department of Medical Biophysics,University of Toronto, 6Princess Margaret Cancer Center,University Health Network, 7Techna Institute,University Health Network, 8Department of Surgery,University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery,University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center,University Health Network, 11Princess Margaret Cancer Center,University Health Network
Chapters
- 00:04Title
- 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
- 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
- 03:27Surgical Field Preparation
- 04:17Laparotomy and Uterine Horn Identification
- 05:31Myometrial VX2 Cell Inoculation
- 06:21Surgical Incision Closure and Post-Operative Care
- 08:03Results: VX2 Tumor Establishment and Characterization
- 09:09Conclusion
This protocol presents a standardized method to grow VX2 cells in culture and to create an orthotopic VX2 model of endometrial cancer with retroperitoneal lymph node metastases in rabbits. Orthotopic endometrial cancer models are important for the pre-clinical study of novel imaging modalities for the diagnosis of lymph node metastases.
