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Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
DOI:

06:27 min

April 17, 2019

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Chapters

  • 00:04Title
  • 00:45Mouse Embryo Collection and Dissection
  • 02:39Whole-mount Epifluorescence Imaging
  • 03:23Genotyping
  • 04:34Results: Cardiac Chamber Development During Mouse Embryogenesis in MLC-2v-tdTomato Knock-in Mice
  • 06:02Conclusion

Summary

Automatic Translation

We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

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