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Modello Xenotrapianto eterogeneo derivato dal paziente del cancro pancreatico utilizzando larve di pesce zebra come ospiti per la valutazione comparativa del farmaco
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Modello Xenotrapianto eterogeneo derivato dal paziente del cancro pancreatico utilizzando larve di pesce zebra come ospiti per la valutazione comparativa del farmaco

Article DOI: 10.3791/59507-v • 09:56 min • April 30th, 2019
April 30th, 2019 •
*1, *2,3, *1, 2,3, 1, 1, 2,3, 2,4,5,6, 1
1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College, Fudan University, 6Pancreatic Cancer Institute, Fudan University
* These authors contributed equally

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Questo protocollo descrive le procedure di ottimizzazione in un modello di xenotrapianto tumorale a doppia fluorescenza basato su virus utilizzando il pesce zebra larva come ospiti. Questo modello xenotrapianto eterogeneo imita la composizione tissutale del microambiente del cancro al pancreas in vivo e funge da strumento più preciso per valutare le risposte ai farmaci in modelli personalizzati zPDX (zebrafish patient-derived xenogrft).

Tags

Ricerca sul cancro Problema 146 Ricerca sul cancro xenotrapianto tumorale pesce zebra carcinoma pancreatico valutazione del farmaco in vivo BCL2L1
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