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Culturing and Measuring Fetal and Newborn Murine Long Bones
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Developmental Biology
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JoVE Journal Developmental Biology
Culturing and Measuring Fetal and Newborn Murine Long Bones

Culturing and Measuring Fetal and Newborn Murine Long Bones

DOI:
10.3791/59509-v

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06:58 min

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April 26, 2019

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1Australian Regenerative Medicine Institute,Monash University

Chapters

  • 00:04Title
  • 00:42Fetal Tibia and Femur Culture and Full Bone Length and Mineralized Region Analysis
  • 04:50Results: Representative Total Bone Length Growth and Mineralized Tibia Measurement and Analysis
  • 06:24Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a method for ex vivo culture of long murine bones at both fetal and newborn stages, suitable for analyzing bone and cartilage development and homeostasis in controlled conditions while recapitulating the in vivo process.

Tags

FetalNewbornMurineLong BonesMetatarsal CultureBone DevelopmentEx Vivo CultureGenetic ModelsLive ImagingPhysical ManipulationPharmacological ManipulationDissectionLimb SeparationBone Culture

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