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Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
DOI:

06:58 min

May 27, 2019

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Chapters

  • 00:04Title
  • 00:54Polymer Isolation and Removal of Contaminants
  • 01:53Precipitation and Lyophilization of the Polymer
  • 03:11Cyanobacterial Exoproteome Isolation
  • 04:57Results: Isolation of Released Carbohydrate Polymers and Exoproteomes from Cyanobacteria Culture Media
  • 06:11Conclusion

Summary

Automatic Translation

Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.

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