01:17Normalization to Determine the Optimal Initial Tension
03:43Phenylephrine-induced Contractions
05:52Results: Optimal Initial Tensions and Output Recordings of the Vasoconstrictor Responses to Phenylephrine in Mesenteric Arteries With or Without Surrounding PVAT
The protocol describes the use of wire myography to evaluate the transmural isometric tension of mesenteric arteries isolated from mice, with special consideration of the modulation by factors released from endothelial cells and perivascular adipose tissues.