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DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DOI:
10.3791/59700-v

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09:12 min

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September 16, 2019

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,
1School of Biosciences,University of Birmingham

Chapters

  • 00:04Title
  • 00:29DNAzyme Design
  • 01:46 S. cerevisiae Growth and RNA Isolation
  • 02:22DNAzyme Digestion
  • 05:07RNA Purification and Electrophoresis
  • 07:27Results: Analysis of Site-specific Methylation with 10-23 and 8-17 DNAzymes
  • 08:21Conclusion

Summary

Automatic Translation

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Here we present a protocol for DNAzyme-dependent cleavage of RNA. This enables fast and site-dependent analysis of RNA 2’-O-methylation. This approach can be used for the preliminary or major assessment of snoRNA activity.

Tags

DNAzymeRNA MethylationSnoRNAYeastMolecular BiologyRNA IsolationRNA AnalysisRNA Digestion10-23 DNAzyme8-17 DNAzymeSaccharomyces Cerevisiae

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