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JoVE Journal
Developmental Biology

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Analysis of Epididymal Protein Synthesis and Secretion
 

Analysis of Epididymal Protein Synthesis and Secretion

Article doi: 10.3791/58308
August 25th, 2018

Summary August 25th, 2018

Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.

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