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Journal / Immunology and Infection / Isolering av lamina propria mononukleære celler…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering av lamina propria mononukleære celler fra murine Colon bruke Kollagenase E
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Isolering av lamina propria mononukleære celler fra murine Colon bruke Kollagenase E

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

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Målet med denne protokollen er å isolere mononukleære celler som ligger i lamina propria i tykktarmen ved enzymatisk fordøyelse av vevet ved hjelp av kollagenase. Denne protokollen gjør det mulig for effektiv isolering av mononukleære celler som resulterer i en enkelt celle suspensjon som i sin tur kan brukes til robuste immunfenotyping.

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Immunologi og infeksjon lymfocytter kolon murine lamina propria kolitt transplantasjon flyt flowcytometri enzymatisk fordøyelse betennelse mononukleære celler
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