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Journal / Immunology and Infection / Reinigung von hochertragreichen extrazellulären…
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Immunology and Infection

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Reinigung von hochertragreichen extrazellulären Vesikelpräparaten weg vom Virus
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Reinigung von hochertragreichen extrazellulären Vesikelpräparaten weg vom Virus

Article DOI: 10.3791/59876-v • 07:15 min • September 12th, 2019
September 12th, 2019 •
*1, *1, 1, 1, 1,2, 3, 4, 5, 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
* These authors contributed equally

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Dieses Protokoll isoliert extrazelluläre Vesikel (EVs) von Virionen mit hoher Effizienz und Ausbeute, indem EV-Fällung, Dichtegradient-Ultrazentrifugation und Partikelerfassung integriert werden, um einen optimierten Workflow und eine Reduzierung des Startens zu ermöglichen. Mengenanforderungen, die zu reproduzierbaren Präparaten für den Einsatz in allen EV-Forschungen führen.

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Immunologie und Infektion Ausgabe 151 extrazelluläre Vesikel Exosom Virus Reinigung Dichtegradient Ultrazentrifugation Niederschlag EV-anreichernde Nanopartikel
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