JoVE Journal
Biology
Biology
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Summary
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由于NADH和fura-2类似物的激发和发射波长的光谱重叠,在[Ca2]的定量测量中,活细胞中两种化学物质的信号干扰是不可避免的。因此,开发了一种新型的NADH信号干扰在线校正方法,以测量[Ca2]。