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实时成像检测方法的评估钙的作用<sup> 2 +</sup>和鞘磷脂酶在成孔毒素伤口的修复
JoVE Journal
Biology
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JoVE Journal
Biology
Live Imaging Assay for Assessing the Roles of Ca
2+
and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
Please note that all translations are automatically generated.
Click here for the English version.
实时成像检测方法的评估钙的作用<sup> 2 +</sup>和鞘磷脂酶在成孔毒素伤口的修复
DOI:
10.3791/50531-v
•
18:25 min
•
August 25, 2013
•
Christina Tam
,
Andrew R. Flannery
,
Norma Andrews
1
Department of Cell Biology and Molecular Genetics
,
University of Maryland
Chapters
00:05
Title
01:41
Transcriptional Silencing of ASM
04:18
Preparation of Reagents for Live Microscopy
05:29
Live Cell Imaging of FM1-43 Influx into SLO Treated and Untreated Cells
10:21
Quantification of FM1-43 Influx into Cells
14:03
Results: Ca
2+
and Sphingomyelinase are Involved in Plasma Membrane Repair
17:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞暴露于所述亲脂性染料FM1-43的实时成像可以精确测定由该成孔毒素从质膜除去动力学。这是一个敏感的测定法可以用于评估要求的Ca
2 +,
鞘磷脂和其它因素对质膜修复。
Tags
Live Imaging
Ca2+
Sphingomyelinase
Pore-forming Toxin
Plasma Membrane Repair
FM1-43
Streptolysin O
Exocytosis
Endocytosis
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