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测量细胞内Ca<sup> +</sup在人类精子的变化采用4项技术:传统的荧光,停流荧光,流式细胞术及单细胞成像
JoVE Journal
Biology
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JoVE Journal Biology
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
DOI:

19:26 min

May 24, 2013

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Chapters

  • 00:05Title
  • 02:38Sperm Sample Preparation by the Swim-up Method
  • 04:52Fluorescent Dye Loading for Ca2+ Measurements
  • 05:41Conventional Fluorometry
  • 06:59Stopped Flow Fluorometry
  • 08:30Flow Cytometry
  • 09:40Single Cell Imaging
  • 12:11Results: Progesterone Induces Intracellular Ca2+ Changes in Human Sperm Detected by Fluorometric Techniques
  • 17:59Conclusion

Summary

Automatic Translation

细胞内Ca<sup> +</sup>动力学是非常重要的,在精子生理学和Ca<sup> +</sup>敏感的荧光染料构成一个多功能的工具来研究它们。人口实验(荧光和停流荧光)和单细胞实验(流式细胞仪和单细胞成像)是用来跟踪时空的[Ca<sup> +</sup>]在人类精子细胞中的变化。

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