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Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents
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Biology
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Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

DOI:
10.3791/59884-v

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08:30 min

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January 31, 2020

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, , ,
1Department of Pharmaceutical Sciences, College of Pharmacy,University of Tennessee Health Science Center, 2Department of Urology,Medical University of South Carolina, 3Department of Urology, College of Medicine,University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine,University of Tennessee Health Science Center

Chapters

  • 00:04Introduction
  • 01:45Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
  • 03:28Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
  • 05:52Results: Investigation of Ion Channel Properties in Isolated DSM Cells
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for preparation of the single freshly isolated detrusor smooth muscle cells from human urinary bladder specimens employing a two-step enzymatic procedure. The obtained viable DSM cells can be studied by various single cell techniques including the described amphotericin-B patch-clamp electrophysiology to reveal physiological and pharmacological properties.

Tags

Human Detrusor Smooth Muscle CellsBladder HistologySingle Cell IsolationEnzymatic DigestionPatch-clamp ElectrophysiologyTRPM4 ChannelIon Channel Characterization

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